CST, 35126S, Periostin (F7U5N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying periostin mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Periostin (F7U5N) Rabbit Monoclonal Antibody (CST #35126) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:1500 - 1:6000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 96408 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Periostin (F7U5N) Rabbit Monoclonal Antibody recognizes endogenous levels of total periostin protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to mouse periostin protein. Background Periostin (POSTN, OSF2) is a secretory matricellular protein that belongs to the fasciclin family. The protein has multiple functional domains that interact with different extracellular matrix (ECM) components (1-2). The N-terminal EMI domain of periostin interacts with fibronectin as well as collagens and lysyl oxidase. The middle part of the protein consists of four FAS 1 (fasciclin) domains which are responsible for the protein association with tenascin-C and CCN3. The C-terminal CTD domain assumes an inhibitory effect on FAS1 domain function. Lastly, the C-terminal HBD domain mediates proteoglycan association (1-2). Periostin has been shown to mediate important cellular processes such as ECM stemness, mechanical stress response, collagen cross-linking and fibrogenesis, cell proliferation, survival, and adhesion (1-3). It is an important modulator in multiple disease developments and has been frequently used as a biomarker for cancer, fibrosis, inflammation, and tissue wound healing (4-7). Alternate Names A630052E07Rik; AI747096; OSF; OSF-2; Osf2; osteoblast specific factor 2 (fasciclin I-like); Osteoblast-specific factor 2; peri; Periostin; periostin-like factor; periostin, osteoblast specific factor; PLF; PN; Postn Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG