CST, 35214T, FATP4/SLC27A4 (F4Y5M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLC27A4/FATP4. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, FATP4/SLC27A4 (F4Y5M) Rabbit Monoclonal Antibody (CST #35214) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FATP4/SLC27A4 (F4Y5M) Rabbit Monoclonal Antibody recognizes endogenous levels of total FATP4/SLC27A4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FATP4/SLC27A4 protein. Background Long-chain fatty acid transport protein 4 (FATP4), also known as solute carrier family 27 member 4 (SLC27A4), is a transmembrane protein in the endoplasmic reticulum (ER) (1). It is an acyl-CoA synthetase and has higher enzymatic activity toward saturated and monounsaturated very long-chain fatty acids (1,2). Studies show that FATP4/SLC27A4 promotes fatty acid uptake by endothelial cells through its acyl-CoA synthetase activity. This enzymatic activity requires ATP produced in mitochondria that are positioned closely to the ER (3). In addition, during starvation of myoblasts, perilipin-5 on lipid droplets interacts with FATP4/SLC27A4 on mitochondria to promote fatty acid transport from lipid droplets to mitochondria (4). Alternate Names ACSVL4; Arachidonate--CoA ligase; FATP-4; FATP4; Fatty acid transport protein 4; IPS; Long-chain fatty acid transport protein 4; Long-chain-fatty-acid--CoA ligase; S27A4; SLC27A4; solute carrier family 27 (fatty acid transporter), member 4; Solute carrier family 27 member 4; Very long-chain acyl-CoA synthetase 4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG