CST, 3522S, Phospho-PNK1 (Ser114/Thr118) Antibody

Polyclonal Antibody for studying Pnk1 (Ser114/Thr118) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-PNK1 (Ser114/Thr118) Antibody (CST #3522) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-PNK1 (Ser114/Thr118) Antibody detects endogenous levels of PNK1 only when phosphorylated on Ser114 and Thr118. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser114 and Thr118 of human PNK1. Antibodies are purified by protein A and peptide affinity chromatography. Background PNK (polynucleotide kinase) is a DNA repair enzyme that participates in single strand break repair and non-homologous end rejoining (NHEJ) for double strand breaks. PNK possesses a 5'-DNA kinase activity and a 3'-DNA phosphatase activity (1,2). It has three domains, a C-terminal kinase domain, a central phosphatase domain, and an N-terminal forkhead associated (FHA) domain that is responsible for protein-protein interactions. Reduction in expression of PNK by RNAi sensitizes cells to ionizing radiation and topoisomerase I inhibitors (3) Phosphorylation of PNK1 at Ser114/ Thr118 was independently identified at Cell Signaling Technology (CST) using PhosphoScan , CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of PNK1 at Ser114/ Thr118 was observed in extracts of various cell lines following UV treatment. For additional information please visit PhosphoSitePlus , CST's modification site knowledgebase, at www.phosphosite.org. Alternate Names 2'(3')-polynucleotidase; AOA4; Bifunctional polynucleotide phosphatase/kinase; CMT2B2; DNA 5'-kinase/3'-phosphatase; EIEE10; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP); MCSZ; PNK; Pnk1; PNKP; Polynucleotide 3'-phosphatase; Polynucleotide 5'-hydroxyl-kinase; polynucleotide kinase 3-prime-phosphatase; polynucleotide kinase 3'-phosphatase; Polynucleotide kinase-3'-phosphatase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 59 SOURCE: Rabbit