CST, 3523S, RalB Antibody

Polyclonal Antibody for studying RalB. Validated for Western Blotting. Highly specific and rigorously validated in-house, RalB Antibody (CST #3523) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RalB Antibody detects endogenous levels of total RalB protein. This antibody might also react with overexpressed RalA protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu142 of human RalB protein. Antibodies are purified by peptide affinity chromatography. Background RalA and RalB are members of the Ras family of small GTPases and are highly homologous in protein sequence. The functions of RalA and RalB are distinct yet overlapping. By binding to various effector proteins, RalA and RalB serve as important GTP sensors for exocytosis and membrane trafficking (1-3). RalA is required for Ras-related tumorigenesis (4) and RalB is important for tumor survival (5). In addition to tumor formation, Ral proteins also play a role in cancer cell migration and metastatic tumor invasion (6,7). Alternate Names GTP binding protein; GTP binding protein); RALB; RALB Ras like proto-oncogene B; RAS like proto-oncogene B; ras related GTP binding protein B; RAS-like protein B; Ras-related protein Ral-B; v-ral simian leukemia viral oncogene homolog B (ras related Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 26 SOURCE: Rabbit