CST, 35264SF, PPAR gamma (C26H12) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PPAR-gamma. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, PPAR gamma (C26H12) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #35264) is ready to ship. Product Usage Information This product is the carrier free version of product #2435. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2435 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PPAR gamma (C26H12) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total PPARγ protein. Non-specific labeling may be observed by immunofluorescence in mitotic cells. Species Reactivity: Human, Mouse Source / Purification PPARγ (C26H12) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Asp69 of human PPARγ. Background Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator (1). PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage (2). Besides its role in mediating adipogenesis and lipid metabolism (2), PPARγ also modulates insulin sensitivity, cell proliferation and inflammation (3). PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84 (4,5). Alternate Names CIMT1; GLM1; NR1C3; Nuclear receptor subfamily 1 group C member 3; peroxisome proliferative activated receptor gamma; peroxisome proliferator activated receptor gamma; peroxisome proliferator-activated nuclear receptor gamma variant 1; Peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor gamma 1; peroxisome proliferator-activated receptor-gamma 5; peroxisome proliferator-activated receptor-gamma splicing; PPAR gamma; PPAR-gamma; PPARG; PPARG1; PPARG2; PPARG5; PPARgamma Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 53, 57 Source/Isotype: Rabbit IgG