CST, 3528S, CD31 (PECAM-1) (89C2) Mouse Monoclonal Antibody

Monoclonal Antibody for studying PECAM-1. Validated for WB,IP,IHC,IF. Available in 3 sizes. Highly specific and rigorously validated in-house, CD31 (PECAM-1) (89C2) Mouse Monoclonal Antibody (CST #3528) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85873 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CD31 (PECAM-1) (89C2) Mouse Monoclonal Antibody detects endogenous levels of total CD31 protein. This antibody does not cross-react with other related proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human CD31 protein. Background CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3). Alternate Names adhesion molecule; CD31; CD31 antigen; CD31/EndoCAM; EndoCAM; FLJ58394; GPIIA'; PECA1; PECAM-1; PECAM-1, CD31/EndoCAM; PECAM1; platelet and endothelial cell adhesion molecule 1; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Mouse IgG1