CST, 35368S, CD7 Antibody

Polyclonal Antibody for studying CD7. Validated for Western Blotting. Highly specific and rigorously validated in-house, CD7 Antibody (CST #35368) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD7 Antibody recognizes endogenous levels of total CD7 protein. This antibody cross-reacts with an unidentified protein of 90 kDa in some cell extracts. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile69 of human CD7 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background CD7 is a type-I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is one of the earliest surface markers to be expressed on the surface of developing T cells, and its expression is maintained throughout maturation of multiple T cell subsets and NK cells (1-3). Engagement of CD7 through binding its ligand, SECTM1, has been shown to promote tyrosine phosphorylation of its cytoplasmic domain, recruitment of PI3K, and delivery of costimulatory signals for T cell activation (4-6). While CD7 is expressed on normal T cells, it is also highly expressed in a variety of T cell malignancies, which has poised it as a potential target of immunotherapy (7-9). Alternate Names CD7; CD7 antigen; CD7 antigen (p41); CD7 molecule; GP40; LEU-9; p41 protein; T-cell antigen CD7; T-cell leukemia antigen; T-cell surface antigen Leu-9; Tp40; TP41 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 38-40 SOURCE: Rabbit