CST, 35393S, ARID5A (F7T9F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ARID5A. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ARID5A (F7T9F) Rabbit Monoclonal Antibody (CST #35393) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ARID5A (F7T9F) Rabbit Monoclonal Antibody recognizes endogenous levels of total ARID5A protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu154 of human ARID5A protein. Background AT-rich interactive domain-containing protein 5A (ARID5A) is a member of the ARID family, which includes 15 distinct human proteins. ARID is a nucleic acid binding domain that has a helix-turn-helix motif and is conserved in all eukaryotes (1,2). ARID5A expression is seen in many immune cells, including macrophages, CD4 T cell subsets TH1 and TH17, as well as stromal cells and keratinocytes (2). During inflammation, ARID5A is translocated from the nucleus to the cytoplasm and stabilizes a variety of mRNA transcripts, including IL-6, Stat3, OX40, T-bet, and IL-17, contributing to the inflammatory response and a variety of inflammatory diseases. TLR4-activated NF-kB and MAPK pathways are involved in regulating ARID5A expression (3). ARID5A plays an important role in resistance to chimeric antigen receptor (CAR)-T cell therapy by stabilizing IDO1 mRNA and the upregulation of IDO1 expression. Elevated IDO1 expression levels facilitate the conversion of tryptophan to kynurenine, which contributes to CAR-T cell exhaustion (4). Alternate Names ARI5A; ARID domain-containing protein 5A; ARID5A; AT rich interactive domain 5A; AT rich interactive domain 5A (MRF1-like); AT-rich interaction domain 5A; AT-rich interactive domain-containing protein 5A; Modulator recognition factor 1; modulator recognition factor I; MRF-1; MRF1; RFVG5814; RP11-363D14 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG