CST, 35396S, SMCR8 Antibody

Polyclonal Antibody for studying SMCR8. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, SMCR8 Antibody (CST #35396) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SMCR8 Antibody recognizes endogenous levels of total SMCR8 protein. A band of unknown origin is detected at around 70 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala540 of human SMCR8 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background SMCR8 (Smith-Magenis chromosome region 8) was characterized as a member of the DENN (differentially expressed in normal and neoplastic cells) domain family that typically function in intracellular membrane trafficking as guanine exchange factors for Rab GTPases (1). Deletion of the gene was found in patients having Smith-Magenis syndrome, a rare developmental disorder (2,3). SMCR8 interacts with another DENN protein C9orf72, and the WD repeat containing protein WDR41, that function as a guanine exchange factor for Rab8a and Rab39b and regulate autophagy (4). Interestingly, C9orf72 is also associated with neurodegenerative diseases including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) (5,6). C9orf72 and SMCR8 were found to have multifaceted roles in autophagy and lysosomal signaling (reviewed 7). SMCR8 can be phosphorylated by kinases with roles in autophagy including TBK1 and ULK1 (4). Indeed, the complex containing C9orf72, SMCR8, and WDR41 associates with the ULK1 complex essential for initiation of autophagy (8). Furthermore, SMCR8 was found in an RNAi screen as a regulator of ULK1 activity and both initiation and maturation of autophagosomes (9). Knockdown of SMCR8 has led to inflammatory and autoimmune phenotypes as well as inhibition of autophagy, elevated activity of mTORC1 and Akt, and decreased lysosomal biogenesis (10-13). Alternate Names DENND8A; FLJ34716; FLJ60657; Guanine nucleotide exchange protein SMCR8; SMCR8; SMCR8-C9orf72 complex subunit; Smith-Magenis syndrome chromosomal region candidate gene 8 protein; Smith-Magenis syndrome chromosome region, candidate 8 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 140-150 SOURCE: Rabbit