CST, 3541S, Phospho-NPM1 (Thr199) Antibody

Polyclonal Antibody for studying NPM1 (Thr199) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-NPM1 (Thr199) Antibody (CST #3541) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-NPM1 (Thr199) Antibody detects endogenous levels of NPM1 only when phosphorylated at Thr199. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr199 of human NPM1. Antibodies are purified by protein A and peptide affinity chromatography. Background Nucleophosmin (NPM1), also known as B23, numatrin, or NO38, is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication, and molecular chaperoning activities (1,2). The gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM1 portion contributes to transformation by providing a dimerization domain, which activates the fused kinase (3,4). NPM associates with unduplicated centrosomes and is a direct substrate of Cdk2-cyclin E in centrosome duplication (4). Upon phosphorylation at Thr199 by Cdk2-cyclin E, NPM dissociates from centrosomes, and this dissociation is a prerequisite step for centrosome to initiate duplication (5). Alternate Names B23; MGC104254; NPM; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; Numatrin; testicular tissue protein Li 128 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 38 SOURCE: Rabbit