CST, 3543S, Bit1 Antibody

Polyclonal Antibody for studying BIT1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Bit1 Antibody (CST #3543) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Bit1 Antibody detects endogenous levels of total human Bit1 protein. This antibody also detects overexpressed bovine Bit1 and may detect overexpressed levels of mouse and rat Bit1. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues within a central region of Bit1. Antibodies were purified by protein A and peptide affinity chromatography. Background Bit1 is a mitochondrial protein discovered as a regulator of anoikis, a process of apoptosis triggered by the loss of cell adhesion to the extracellular matrix (1,2). Like several other mitochondrial apoptotic regulators (Cytochrome c, Smac, HtrA2, AIF, EndoG), Bit1 is released into the cytoplasm during apoptosis (1,3). Cytoplasmic Bit1 associates with the Groucho family of transcriptional regulator AES (1,4). Interaction between Bit1 and AES promotes apoptosis, and this complex is suppressed by integrin mediated cell attachment. Alternate Names Bcl-2 inhibitor of transcription 1; BIT1; CFAP37; CGI-147; cilia and flagella associated protein 37; FLJ32471; IMNEPD; peptidyl-tRNA hydrolase 2; Peptidyl-tRNA hydrolase 2, mitochondrial; PTH; PTH 2; PTH2; PTRH2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 19, 21 SOURCE: Rabbit