CST, 3545S, Nod1 Antibody

Polyclonal Antibody for studying NOD1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Nod1 Antibody (CST #3545) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Nod1 Antibody detects endogenous levels of Nod1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of Nod1 protein. Antibodies were purified by protein A and peptide affinity chromatography. Background Nod1/CARD4 is a cytosolic protein structually related to Apaf-1 and plant drug-resistance proteins that has been implicated in apoptosis and inflammatory responses to certain pathogenic bacteria (1-3). It contains an amino-terminal caspase recruitment domain (CARD) that is linked to a central nucleotide-binding domain (NBD; also known as a NOD domain) and is followed by carboxy-terminal leucine-rich repeats (LRR) (1). Like Apaf-1, Nod1 induces apoptosis by a CARD/NBD-dependent activation of caspase-9 (1). The primary function of Nod1 is thought to be as a sensor for certain pathogenic microbes and triggering inflammatory responses including the activation of NF-κB and JNK pathways (4-6). The LRR of Nod1 appears to be involved in recognition of microbial components and the CARD domain induces NF-κB activation in cooperation with the CARD containing kinase, RICK/RIP2/CARDIAK (1,5,6). Mutations in Nod1 have been linked increased susceptibility to asthma (7) and inflammatory bowel disease (8). Alternate Names CARD4; caspase recruitment domain family, member 4; Caspase recruitment domain-containing protein 4; CLR7.1; NLR family, CARD domain containing 1; NLRC1; NOD1; nucleotide binding oligomerization domain containing 1; nucleotide-binding oligomerization domain containing 1; Nucleotide-binding oligomerization domain-containing protein 1; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 95 SOURCE: Rabbit