CST, 3546S, Arf6 Antibody

Polyclonal Antibody for studying ARF6. Validated for Western Blotting. Highly specific and rigorously validated in-house, Arf6 Antibody (CST #3546) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Arf6 Antibody detects endogenous levels of total Arf6 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly165 of human Arf6 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background ADP-ribosylation factor (Arf) proteins are low molecular weight GTP binding proteins that belong to the Ras GTPase superfamily (1). Arf proteins are grouped into three distinct classes based on amino acid sequence and structural similarity, with Arf6 as the single class III protein to date. Arf6 is localized mainly to the plasma membrane and endosomes (1,2). This small GTPase interacts with PIP5K, PLD and Rac1, proteins important in lipid metabolism and actin regulation. Arf6 function depends upon its cycling between GDP- and GTP-bound states, which is regulated by associated GAP and GEF factors (3,4). Plasma membrane-associated Arf6 appears to play several functions during the many steps of membrane trafficking, including regulating membrane receptor internalization in both clathrin-dependent and independent pathways, endosomal recycling, and proximal actin reorganization and remodeling (5,6). Alternate Names ADP ribosylation factor 6; ADP-ribosylation factor 6; ARF6; DKFZp564M0264 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 19 SOURCE: Rabbit