CST, 3556T, Phospho-RSK2 (Ser227) (D53A11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RSK2 (Ser227) phosphate/RSK1 (Ser221) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-RSK2 (Ser227) (D53A11) Rabbit Monoclonal Antibody (CST #3556) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-RSK2 (Ser227) (D53A11) Rabbit Monoclonal Antibody detects endogenous levels of RSK2 only when phosphorylated at Ser227. This antibody shows cross-reactivity with RSK1 when phosphorylated at the homologous serine residues. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser227 of human RSK2. Background The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). PDK1 phosphorylates Ser227 in the activation loop of the amino-terminal kinase domain of RSK2, leading to substantial activation of the kinase and . The constitutively active PDK1 cooperates with ERK in the activation of RSK following the exposure of cells to growth factors or UV-light (4, 5). Alternate Names 90 kDa ribosomal protein S6 kinase 1; 90 kDa ribosomal protein S6 kinase 3; CLS; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); epididymis secretory sperm binding protein; HU-1; HU-3; Insulin-stimulated protein kinase 1; ISPK-1; ISPK1; KS6A1; KS6A3; MAP kinase-activated protein kinase 1a; MAP kinase-activated protein kinase 1b; MAPK-activated protein kinase 1a; MAPK-activated protein kinase 1b; MAPKAP kinase 1a; MAPKAP kinase 1b; MAPKAPK-1a; MAPKAPK-1b; MAPKAPK1; MAPKAPK1A; MAPKAPK1B; mental retardation, X-linked 19; MRX19; p90-RSK 1; p90-RSK 3; p90-RSK2; p90RSK; p90RSK1; p90RSK3; p90S6K; pp90RSK2; ribosomal protein S6 kinase A1; ribosomal protein S6 kinase A3; ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase alpha-3; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 3; Ribosomal S6 kinase 1; Ribosomal S6 kinase 2; RPS6KA1; RPS6KA3; RSK; RSK-1; RSK-2; RSK1; RSK2; S6K-alpha 1; S6K-alpha-1; S6K-alpha-3; S6K-alpha3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG