CST, 3558T, LDHA/LDHC (C28H7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LDH-A/LDH-C. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, LDHA/LDHC (C28H7) Rabbit Monoclonal Antibody (CST #3558) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31037 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity LDHA/LDHC (C28H7) Rabbit Monoclonal Antibody detects endogenous levels of total LDHA and LDHC proteins. Species cross-reactivity for IHC-P is human only. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr307 of human LDHA protein. Background Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD . When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD , which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain. While LDHA is found primarily in muscle and kidney, LDHC is a testis-specific isoform (3). Both proteins are associated with human disease. Mutations in the corresponding LDHA gene are associated with LDHA deficiency, which is characterized by muscle stiffness following exercise and uterine stiffness during pregnancy (4). Abnormal LDHC expression is associated with several forms of cancer (5). Alternate Names Cancer/testis antigen 32; cell proliferation-inducing gene 19 protein; CT32; epididymis secretory sperm binding protein Li 133P; GSD11; HEL-S-133P; L-lactate dehydrogenase A chain; L-lactate dehydrogenase C chain; lactate dehydrogenase A; lactate dehydrogenase C; lactate dehydrogenase c variant 1; lactate dehydrogenase c variant 3; lactate dehydrogenase c variant 4; lactate dehydrogenase c variant 5; lactate dehydrogenase C4; lactate dehydrogenase M; LDH muscle subunit; LDH testis subunit; LDH-A; LDH-C; LDH-M; LDH-X; LDH1; LDH3; LDHA; LDHC; LDHM; LDHX; MGC111073; PIG19; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59; testis secretory sperm-binding protein Li 234P Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG