CST, 35602T, FHL2 (E5E3D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FHL2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FHL2 (E5E3D) Rabbit Monoclonal Antibody (CST #35602) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FHL2 (E5E3D) Rabbit Monoclonal Antibody recognizes endogenous levels of total FHL2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu14 of human FHL2 protein. Background Four and a half LIM-only protein 2 (FHL2) is a member of the LIM domain protein family that interacts with a large number of proteins, including receptors, signal transducers, and transcription factors (1). LIM proteins have one or more LIM domains composed of a conserved cysteine-rich module that mediates protein interactions (2). FHL2 is involved in regulating various cellular processes, such as survival, proliferation, migration, and signal transduction in muscle and cardiac tissue (1-3). In immune cells, FHL2 has been shown to play a role in B cell differentiation and maturation in secondary lymphoid organs, and its deletion alters cytokine release and migration of dendritic cells and macrophages (4,5). FHL2 binds FOXO1, resulting in decreased expression of genes targeted by FOXO (6). FHL2 suppresses tumor growth by interacting directly with HIF-1α and repressing its transcriptional activity (7). When upregulated in tumors, FHL2 can also act as an oncoprotein interacting with multiple transcription factors to suppress apoptosis and promote tumor progression (8). For these reasons, FHL2 is being investigated as a tumor therapeutic target. Alternate Names AAG11; aging-associated gene 11; down-regulated in rhabdomyosarcoma LIM protein; DRAL; FHL-2; FHL2; four and a half LIM domains 2; Four and a half LIM domains protein 2; four and a half LIM-domain protein 2; LIM domain protein DRAL; Skeletal muscle LIM-protein 3; SLIM-3; SLIM3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG