CST, 35615T, Tyk2 (E9H4T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tyk2 mouse. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Tyk2 (E9H4T) Rabbit Monoclonal Antibody (CST #35615) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 49385 . Protocol Available protocols: Western Blotting Specificity / Sensitivity Tyk2 (E9H4T) Rabbit Monoclonal Antibody recognizes endogenous levels of total Tyk2 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro371 of mouse Tyk2 protein. Background Tyk2 is a member of the Jak family of protein tyrosine kinases. It associates with and is activated by receptors for many cytokines including IL-13, the IL-6 family, IL-10, and IFN-α and β (1-3). Following ligand binding, Tyk2 is activated by phosphorylation of Tyr1054 and/or Tyr1055 (4). Tyk2 is required for the tyrosine phosphorylation of Stat3 in the IFN-β signaling cascade (5). The role of Tyk2 has been extensively studied in terms of its involvement in immune regulation and pathological significance (reviewed in 6). Deletion of Tyk2 in mice results in increased sensitivity to infection and defective tumor surveillance, but only a partial effect on Type I interferon signaling (7,8). In contrast, a human patient diagnosed with hyper-IgE syndrome having increased susceptibility to various microorganisms was found to have a homozygous mutation of Tyk2 (9). These studies suggest a more critical role of Tyk2 in humans with regards to Type I interferon signaling as well as other cytokines including IL-23, IL-6, and IL-10. Alternate Names JTK1; Non-receptor tyrosine-protein kinase TYK2; Tyk2; tyrosine kinase 2; tyrosine kinase TYK2 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 134 Source/Isotype: Rabbit IgG