CST, 3561S, CCT2 Antibody

Polyclonal Antibody for studying CCT2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CCT2 Antibody (CST #3561) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CCT2 Antibody detects endogenous levels of total CCT2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human CCT2. Antibodies are purified by protein A and peptide affinity chromatography. Background CCT2 is one of eight largely unrelated subunit proteins found in a protein chaperone complex known as the chaperonin-containing TCP-1 (CCT) or TRiC complex. The CCT complex is an abundanct cytoslic component that is credited with helping newly synthesized polypeptides adopt the correct conformation (1). Proteins that fold and assemble with the help of CCT include the cytoskeletal proteins actin and tubulin as well as up to 15% of newly synthesized eukaryotic proteins (2). CCT2 is the β-subunit of the chaperone complex and is one of several CCT proteins that exhibit increased expression in response to stress. This implies that the CCT complex helps cells recover from protein damage by assisting in protein folding and assembly (3). CCT subunit levels also change throughout the cell cycle, with lower proteins levels (and reduced chaperone activity) found during induced cell cycle arrest during at M phase (4). Each CCT subunit is thought to perform a specific function during protein folding and assembly (5); CCT2 exhibits both actin and tubulin binding activities (6,3) but the exact molecular function on this subunit remains uncertain. Alternate Names 99D8.1; CCT-beta; CCT2; CCTB; chaperonin containing t-complex polypeptide 1, beta subunit; chaperonin containing t-complex polypeptide 1, subunit 2; chaperonin containing TCP1 subunit 2; chaperonin containing TCP1, subunit 2; chaperonin containing TCP1, subunit 2 (beta); epididymis secretory sperm binding protein Li 100n; HEL-S-100n; MGC142074; MGC142076; PRO1633; T-complex protein 1 subunit beta; T-complex protein 1, beta subunit; TCP-1-beta; TCPB Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 54 SOURCE: Rabbit