CST, 35621SF, PILRA (E6H7K) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PILRA mouse. Validated for WB,IF,IF,F,F. Highly specific and rigorously validated in-house, PILRA (E6H7K) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #35621) is ready to ship. Product Usage Information This product is the carrier free version of product #79482. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 79482 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PILRA (E6H7K) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total mouse PILRA protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse PILRA protein. Background Paired immunoglobulin-like type 2 receptor alpha (PILRA) is an inhibitory surface receptor expressed by myeloid cells and other tissues, including microglia in the central nervous system, regulating immune cells and inflammation (1). It functions by recruiting cytoplasmic phosphatases like PTPN6/SHP-1 and PTPN11/SHP-2, blocking downstream signal transduction via their SH2 domains (2). Furthermore, PILRA plays a role as an entry co-receptor for herpes simplex virus-1 via binding of viral glycoprotein B1 (3). More recently, mutations in the gene have been implicated in Alzheimer's disease (AD) (1,4). Recent large-scale GWAS have linked the risk of developing late-onset AD in humans harboring rare variants of microglial immunoreceptors such as TREM2, CD33, and PILRA (5). Alternate Names AV021745; Cell surface receptor FDF03; FDF03; Inhibitory receptor PILR-alpha; OTTMUSP00000026272; paired immunoglobin-like type 2 receptor alpha; Paired immunoglobulin-like type 2 receptor alpha; Pilra Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG