CST, 35644S, CHMP4B (E2L7M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CHMP4B. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CHMP4B (E2L7M) Rabbit Monoclonal Antibody (CST #35644) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CHMP4B (E2L7M) Rabbit Monoclonal Antibody recognizes endogenous levels of total CHMP4B protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn219 of human CHMP4B protein. Background CHMP4B, the human homologue of yeast Snf7, interacts with Alix and is a core component of the ESCRT-III complex (endosomal sorting for transport complex III) (1,2). The ESCRT system is comprised of multiple protein complexes denoted as ESCRT-0, -I, -II, -III, and VPS4 ATPase that together contribute to a number of membrane fission events, including multivesicular body (MVB) and exosome formulation, viral budding, abscission during cytokinesis, and plasma membrane repair (3,4). Interestingly, it was found that the ESCRT-III complex, including CHMP4B is recruited to the plasma membrane during the regulated process of necrosis, necroptosis, and thereby controls membrane integrity sustaining these cells and allowing continued signaling and cytokine/chemokine secretion (5). Elevated expression of CHMP4B has been associated with neuronal apoptosis following intracerebral hemorrhage (6). In addition, high CHMP4B expression was associated with increased proliferation and resistance to doxorubicin in hepatocellular carcinoma (7). Alternate Names C20orf178; Charged multivesicular body protein 4b; CHM4B; CHMP4A; CHMP4B; chromatin modifying protein 4B; Chromatin-modifying protein 4b; CTPP3; CTRCT31; dJ553F4.4; hSnf7-2; hVps32-2; SHAX1; SNF7; SNF7 homolog associated with Alix 1; Snf7 homologue associated with Alix 1; SNF7-2; Vacuolar protein sorting-associated protein 32-2; vacuolar protein-sorting-associated protein 32-2; Vps32-2; VPS32B Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG