CST, 35672S, CRMP-2 (D8L6V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CRMP-2. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, CRMP-2 (D8L6V) Rabbit Monoclonal Antibody (CST #35672) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CRMP-2 (D8L6V) Rabbit Monoclonal Antibody recognizes endogenous levels of total CRMP-2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lle546 of human CRMP-2 protein. Background Collapsin Response Mediator Protein-2 (CRMP-2) is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5). Alternate Names Collapsin response mediator protein 2; collapsin response mediator protein hCRMP-2; CRMP-2; CRMP2; DHPRP2; dihydropyrimidinase like 2; dihydropyrimidinase-like 2; dihydropyrimidinase-like 2 long form; Dihydropyrimidinase-related protein 2; DPYL2; DPYSL2; DRP-2; DRP2; N2A3; ULIP-2; ULIP2; Unc-33-like phosphoprotein 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG