CST, 35676SF, Olig1 (E3T6D) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying OLIG1 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Olig1 (E3T6D) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #35676) is ready to ship. Product Usage Information This product is the carrier free version of product #15849. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 15849 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Olig1 (E3T6D) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Olig1 protein. Non-specific plasma membrane staining was observed in a mouse model of B cell lymphoma and spotty cytoplasmic staining of late spermatids was observed in mouse testis by immunohistochemistry. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val26 of mouse Olig1 protein. Background Oligodendrocyte transcription factor 1 (Olig1) is a basic helix-loop-helix (bHLH) transcription factor expressed in developing and mature oligodendrocytes. Olig1 is involved in oligodendrocyte Specification, differentiation, and myelination and plays a crucial role in promoting the commitment of oligodendrocyte progenitor cells (1). Olig1 has also been shown to play an important role in remyelination after injury (2). Because of its role in development and myelination, Olig1 may play a role in neurodevelopmental disorders like Down syndrome and autism spectrum disorder and myelinating diseases like multiple sclerosis (3,4). Alternate Names AI836478; AW494459; Bhlhb; Bhlhb6; bHLHe2; bHLHe21; Olg; Olg-1; Olig1; Oligo1; Oligodendrocyte transcription factor 1; Oligodendrocyte-specific bHLH transcription factor 1; OTTMUSP00000021401 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 27 Source/Isotype: Rabbit IgG