CST, 35695S, SOAT1 Antibody

Polyclonal Antibody for studying SOAT1. Validated for Western Blotting. Highly specific and rigorously validated in-house, SOAT1 Antibody (CST #35695) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SOAT1 Antibody recognizes endogenous levels of total SOAT1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SOAT1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background SOAT1 (Sterol O-acyltransferase 1; ACAT1) is an O-acyltransferase that functions in the endoplasmic reticulum (ER) to catalyze the formation of cholesterol esters from free cholesterol and long chain fatty acyl-coenzyme A. The cholesterol esters are incorporated into cytoplasmic lipid droplets, thereby preventing excess free cholesterol from inducing lipid-mediated cell toxicity, including ER stress (1). Research studies have shown that pharmacological inhibition of SOAT1 in tumor cells induced lipid-mediated cell toxicity that suppressed tumor cell growth and promoted tumor cell apoptosis (2,3). Pharmacological SOAT1 inhibition was also shown to stimulate autophagy-mediated proteolysis in microglia, leading to enhanced clearance of amyloid peptide Aβ42 (4,5). Collectively, these findings suggest that SOAT1 inhibition may have therapeutic potential in both cancer and Alzheimer's disease. Alternate Names ACACT; ACACT1; ACAT; ACAT-1; ACAT1; acyl-Coenzyme A: cholesterol acyltransferase; Acyl-coenzyme A:cholesterol acyltransferase 1; Cholesterol acyltransferase 1; RP11-215I23.2; SOAT; SOAT1; STAT; sterol O-acyltransferase (acyl-Coenzyme A: cholesterol acyltransferase) 1; Sterol O-acyltransferase 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45 SOURCE: Rabbit