CST, 3580S, Nanog Antibody

Polyclonal Antibody for studying Nanog. Validated for WB,IF,F,ChIP. Highly specific and rigorously validated in-house, Nanog Antibody (CST #3580) is ready to ship. Product Usage Information For optimal ChIP results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:800 Flow Cytometry (Fixed/Permeabilized): 1:400 Chromatin IP: 1:25 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP Specificity / Sensitivity Nanog Antibody detects endogenous levels of total nanog protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid sequence at the N-terminus of human nanog. Antibodies are purified by Protein A and peptide affinity chromatography. Background Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3). Alternate Names hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG; Nanog homeobox Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 SOURCE: Rabbit