CST, 3582T, LDHA (C4B5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LDH-A. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, LDHA (C4B5) Rabbit Monoclonal Antibody (CST #3582) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 43723 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity LDHA (C4B5) Rabbit Monoclonal Antibody detects endogenous levels of total LDHA protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu218 of human LDHA protein. Background Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD . When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD , which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain. Alternate Names cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; GSD11; HEL-S-133P; L-lactate dehydrogenase A chain; lactate dehydrogenase A; lactate dehydrogenase M; LDH muscle subunit; LDH-A; LDH-M; LDH1; LDHA; LDHM; PIG19; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG