CST, 35842S, Spermine oxidase (F7Z4L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMOX. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Spermine oxidase (F7Z4L) Rabbit Monoclonal Antibody (CST #35842) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Spermine oxidase (F7Z4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total spermine oxidase protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro276 of human spermine oxidase protein. Background Polyamines, including spermine, spermidine, and putrescine, are involved in many cellular processes, for example, cell proliferation, differentiation, apoptosis, the synthesis of proteins and nucleic acids, ion channel activity regulation, and the protection from oxidative injury (1). The cellular content of polyamines is tightly regulated; their biosynthesis is catalyzed by different enzymes including spermine synthase (SMS) and spermidine synthase (SRM), while the enzymes N1-acetylpolyamine oxidase (PAOX), spermidine/spermine N1-acetyltransferase 1 (SAT1) and spermine oxidase (SMOX) are involved in polyamine catabolism (1). SMOX is a flavoenzyme that catalyzes the oxidation of spermine to spermidine and is expressed in various tissues, mainly in brain and skeletal muscle (2,3). For several epithelial cancers, strong correlations have been established between chronic inflammation, cancer initiation and progression, and increased levels of SMOX, which lead to amplified H O production, oxidative stress, and DNA damage (4). Mouse models with chronic overexpression of SMOX in cortical neurons show chronic astrocyte dysfunction, oxidative stress, and dysregulation of glutamatergic transmission, leading to epileptic seizures (3). Alternate Names C20orf16; flavin containing amine oxidase; flavin-containing spermine oxidase; FLJ20746; MGC1010; PAO; PAO-1; PAO1; PAOH; PAOh1; Polyamine oxidase 1; putative cyclin G1 interacting protein; SMO; SMOX; Spermine oxidase Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG