CST, 3584S, Phospho-LAT (Tyr220) Antibody

Polyclonal Antibody for studying LAT (Tyr220) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-LAT (Tyr220) Antibody (CST #3584) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-LAT (Tyr220) Antibody detects endogenous levels of LAT when phosphorylated at tyrosine 220. Tyr220 of LAT, long isoform corresponds to Tyr191 of LAT, short isoform. Weak cross-reactivity with phosphorylated tyrosines 171 and 255 of LAT has been observed. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Tyr220 of human LAT. Antibodies are purified by protein A and peptide affinity chromatography. Background LAT, a transmembrane adaptor protein expressed in T, NK, and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4). Alternate Names 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; IMD52; LAT; LAT1; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; p36-38; pp36 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 36, 38 SOURCE: Rabbit