CST, 3585S, PDE5 (P434) Antibody

Polyclonal Antibody for studying PDE5. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, PDE5 (P434) Antibody (CST #3585) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PDE5 (P434) Antibody detects endogenous levels of total PDE5 protein. This antibody cross-reacts with a 35-40 kDa protein of unknown origin. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to central residues of human PDE5. Background The superfamily of phosphodiesterases (PDEs) catalyses the hydrolysis of 3',5'-cyclic nucleotides into the corresponding nucleotide 5'-monophosphates. PDE5 is a cGMP-specific enzyme that contains two cGMP binding sites in its amino-terminal regulatory domain (1). Elevation of cGMP levels causes activation of PKG, which phosphorylates PDE5 at Ser102 (2). Phosphorylation of PDE5 stimulates its enzymatic activity and enhances cGMP binding affinity in the regulatory domain, leading to a decrease in intracellular cGMP levels (3,4). Alternate Names CGB-PDE; cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase; cGMP-binding cGMP-specific phosphodiesterase; cGMP-specific 3',5'-cyclic phosphodiesterase; cGMP-specific phosphodiesterase PDE5A2; cGMP-specific phosphodiesterase type 5A; CN5A; PDE5; PDE5A; PDE5A1; phosphodiesterase 5A; phosphodiesterase 5A, cGMP-specific; phosphodiesterase isozyme 5 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit