CST, 35914T, IMPDH1 (E5Z5F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IMPDH1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IMPDH1 (E5Z5F) Rabbit Monoclonal Antibody (CST #35914) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IMPDH1 (E5Z5F) Rabbit Monoclonal Antibody recognizes endogenous levels of total IMPDH1 protein. This antibody does not cross-react with IMPDH2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val130 of human IMPDH1 protein. Background Inosine-5'-monophosphate dehydrogenase (IMPDH) catalyzes the conversion of IMP to XMP, the first and rate-limiting step of guanine nucleotide biosynthesis (1). The isozymes IMPDH1 and IMPDH2 are encoded by two distinct genes (2). Research studies show that IMPDH2 is overexpressed in glioblastoma. In glioblastoma, IMPDH2-mediated GTP biosynthesis is essential for rRNA and tRNA synthesis and the formation of enlarged and irregular nucleoli. In addition, and double knockout leads to significant inhibition of tumor growth in an orthotopic glioblastoma model (3). Furthermore, IMPDH1 and IMPDH2 expression is upregulated in small cell lung cancer cells with low expression of ASCL1 transcription factor (ASCL1 ). Suppression of IMPDH activity inhibits ASCL1 small cell lung cancer growth (4). Alternate Names DKFZp781N0678; IMDH1; IMP (inosine 5'-monophosphate) dehydrogenase 1; IMP (inosine monophosphate) dehydrogenase 1; IMP dehydrogenase 1; IMPD; IMPD 1; IMPD1; IMPDH 1; IMPDH-I; IMPDH1; inosine monophosphate dehydrogenase 1; Inosine-5'-monophosphate dehydrogenase 1; LCA11; RP10; sWSS2608 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 55, 60 Source/Isotype: Rabbit IgG