CST, 35956SF, Nicastrin (D4F6N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Nicastrin. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Nicastrin (D4F6N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #35956) is ready to ship. Product Usage Information This product is the carrier free version of product #30239. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 30239 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Nicastrin (D4F6N) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Nicastrin protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Nicastrin protein. Background Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (1,2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (3). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to γ-secretase to ensure the correct cleavage process (2,4). Alternate Names anterior pharynx-defective 2; APH2; ATAG1874; KIAA0253; NCSTN; NICA; Nicastrin Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110, 120 Source/Isotype: Rabbit IgG