CST, 3595S, eIF2B epsilon Antibody

Polyclonal Antibody for studying eIF2B-epsilon. Validated for Western Blotting. Highly specific and rigorously validated in-house, eIF2B epsilon Antibody (CST #3595) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity eIF2B epsilon Antibody detects endogenous levels of total eIF2B-epsilon protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the middle of human eIF2B-epsilon. Antibodies are purified by protein A and peptide affinity chromatography. Background Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). eIF2B, a guanine nucleotide exchange factor, is composed of 5 subunits, the largest of which is eIF2B-epsilon (7). Multiple in vivo phosphorylation sites have been identified on eIF2B-epsilon (8). Casein Kinase II can phosphorylate eIF2B-epsilon at Ser717/718 to allow for association with its substrate eIF2. Phosphorylation at Ser544 allows GSK-3 to phosphorylate the key regulatory site Ser540. A fifth eIF2B-epsilon phosphorylation site, Ser466, can be phosphorylated by casein kinase I. Alternate Names CACH; CLE; EI2BE; EIF-2B; eIF-2B GDP-GTP exchange factor subunit epsilon; eIF2B-epsilon; EIF2B5; EIF2BE; EIF2Bepsilon; eukaryotic translation initiation factor 2B subunit epsilon; eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa; LVWM; Translation initiation factor eIF-2B subunit epsilon Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 85 SOURCE: Rabbit