CST, 35969SF, CD70 (E3Q1A) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD70. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD70 (E3Q1A) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #35969) is ready to ship. Product Usage Information This product is the carrier-free version of product #69209. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 69209 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD70 (E3Q1A) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD70 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CD70 protein. Background CD70 is a type II transmembrane glycoprotein and a member of the tumor necrosis factor ligand superfamily (TNFSF), also known as CD27L and TNFSF7. It is normally expressed on medullary thymic epithelial cells. Its expression is induced on activated lymphoid cells (B cells, T cells, and NK cells) and dendritic cells. CD70 is a ligand for CD27, a co-stimulatory receptor that plays an important role in T cell activation and proliferation (1,2). CD70 overexpression has been reported in various tumors such as renal cell carcinoma, glioblastoma, and non-small cell lung carcinoma and it's being actively pursued as a therapeutic target (3-6). Alternate Names CD27 ligand; CD27-L; CD27L; CD27LG; CD70; CD70 antigen; CD70 molecule; Ki-24 antigen; LPFS3; surface antigen CD70; TNFSF7; TNLG8A; tumor necrosis factor (ligand) superfamily, member 7; tumor necrosis factor ligand 8A; Tumor necrosis factor ligand superfamily member 7; tumor necrosis factor ligand superfamily, member 7 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 27, 54, 81 Source/Isotype: Rabbit IgG