CST, 35988S, ICOS (D1K2T™) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying ICOS. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, ICOS (D1K2T^™) Rabbit Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #35988) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity ICOS (D1K2T) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ICOS protein. Background ICOS (Inducible Co-Stimulator, CD278) is a member of the CD28 family that regulates T cell activity and immune responses (1). The ICOS protein contains an extracellular IgV-like domain, a transmembrane domain, and an intracellular domain with a YMFM motif (1-2). ICOS is primarily expressed on activated CD4 and CD8 T cells (1). Upon binding to its ligand, ICOS potentiates the T cell response to antigen through activation of the PI3K signaling pathway (3). In addition to enhancing T cell activation and proliferation, ICOS plays an important role in the regulation of T follicular helper cells (4). Research studies suggest that ICOS is a potential therapeutic target, and could serve as a prognostic biomarker for neoplastic therapy involving CTLA-4 blockade (5-7). Alternate Names Activation-inducible lymphocyte immunomediatory molecule; AILIM; CD278; CVID1; ICOS; inducible costimulator; inducible T cell costimulator; inducible T-cell co-stimulator; Inducible T-cell costimulator; MGC39850; OTTHUMP00000163785; OTTHUMP00000208080 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG