CST, 3605S, DIDO1 Antibody

Polyclonal Antibody for studying DIDO1. Validated for Western Blotting. Highly specific and rigorously validated in-house, DIDO1 Antibody (CST #3605) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DIDO1 Antibody detects endogenous levels of DIDO1 and other isoforms including DIDO2 and DIDO3. An unknown band is detected in HepG2 cells at 35 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr160 of human DIDO1. Antibodies were purified by protein A and peptide affinity chromatography. Background The putative transcription factor DIDO1 (death inducer obliterator 1, also termed DIO-1 or DATF1) contains a pair of zinc finger motifs and is upregulated by apoptotic stimuli. DIDO1 is expressed in the developing limb and may play a role in controlling programmed cell death during development (1-3). Nuclear translocation of DIDO1 during apoptosis is associated with its apoptotic activity (2). Alternative splicing produces the DIDO-1, -2 and -3 isoforms (also termed DIO-1, -2, -3), whose targeted disruption in mice produces a phenotype similar to myelodysplastic/myeloproliferative disease (MPS/MPD) in humans (3). DIDO3, the largest of the splice variants, is associated with the centrosome and plays a role in mitotic checkpoint and chromosome stability (4). Alternate Names BYE1; C20orf158; DATF-1; DATF1; death associated transcription factor 1; death inducer-obliterator 1; Death-associated transcription factor 1; Death-inducer obliterator 1; DIDO1; DIDO2; DIDO3; DIO-1; DIO1; dJ885L7.8; DKFZp434P1115; FLJ11265; hDido1; KIAA0333; MGC16140 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70, 80, 130, 247 SOURCE: Rabbit