CST, 36067T, RanGAP1 (D2T7T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RanGAP1. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, RanGAP1 (D2T7T) Rabbit Monoclonal Antibody (CST #36067) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:100 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RanGAP1 (D2T7T) Rabbit Monoclonal Antibody recognizes endogenous levels of total RanGAP1 protein. This antibody detects unmodified and SUMO-1 modified RanGAP1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RanGAP1 protein. Background Ran GTPase activating protein 1 (RanGAP1) regulates GTP hydrolysis by Ran, which is required for the ability of Ran to regulate nucleocytoplasmic shuttling (1). A significant fraction of cellular RanGAP1 is covalently modified by SUMO-1, which is required for relocalization of RanGAP1from the cytoplasm to the nuclear pore complex and the mitotic spindle (2-5). Research studies demonstrate that RanGAP1 sumoylation is required for stable association of RanGAP1 with RanBP2. Together with the SUMO-conjugating enzyme UBC9/UBE2I, RanGAP1 and RanBP2 are part of a SUMO E3 ligase complex that is implicated in regulating nucleocytoplasmic protein trafficking (6-8). Phosphorylation of RanGAP1 occurs in a cell-cycle-dependent manner and may play a role in regulating RanGAP1 catalytic activity (9,10). Alternate Names Fug1; KIAA1835; MGC20266; RAGP1; Ran GTPase activating protein 1; Ran GTPase-activating protein 1; RANGAP; RANGAP1; SD; segregation distorter homolog; segregation distortion Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 68, 82 Source/Isotype: Rabbit IgG