CST, 36103T, NSUN2 (E6N6I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NSUN2. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, NSUN2 (E6N6I) Rabbit Monoclonal Antibody (CST #36103) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity NSUN2 (E6N6I) Rabbit Monoclonal Antibody recognizes endogenous levels of total NSUN2 protein. This antibody is expected to be nuclear based on function; however, potentially non-specific mitochondrial staining has been observed by non-overlapping antigens in liver by immunofluorescence. This antibody may detect a band of unknown identity at 55 kDa in rat cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys43 of human NSUN2 protein. Background Chemical modifications of RNA regulate many cellular processes. One particular RNA modification, 5-methylcytosine (5-mC), regulates ribosome assembly, translation, and RNA stability (1). In eukaryotes, this modification is added to RNA by the DNA methyltransferase homologue 2 protein (DNMT2; also known as TRDMT1), and also by members of the NOL1/NOP2/SUN domain (NSUN) family of proteins. NSUN proteins are putative S-adenosylmethionine (SAM)-dependent methyltransferases that carry out their enzymatic activity by utilizing two cysteine residues in their active sites (2). There are currently seven known members of this family, consisting of NOP2 (NSUN1) and NSUN2-7. NSUN2 is an 86 kDa member of the NSUN family that is predominantly localized to the nucleus and methylates a variety of tRNAs and other RNA substrates (3). NSUN2 has been found to play a role in the methylation of pre-tRNA , tRNA , tRNA , tRNA , and tRNA (1,4,5). It has also been found to methylate mRNAs and several ncRNAs, such as vtRNAs (6-8). Elevated NSUN2 protein levels have been reported in multiple types of human cancers, including breast, prostate, kidney, bladder, and liver, among others (9). The methyltransferase activity of NSUN2 is reduced upon Aurora B kinase-mediated phosphorylation at Ser139 (10), and there is potential for NSUN2 to be used as a therapeutic target or diagnostic marker for Aurora B linked cancers (9). Alternate Names 5-methycytoisine methyltransferase; cDNA FLJ76184, highly similar to Homo sapiens NOL1/NOP2/Sun domain family, member 2 (NSUN2), mRNA; FLJ20303; hTrm4; MISU; mRNA cytosine C(5)-methyltransferase; MRT5; Myc-induced SUN domain-containing protein; Myc-induced SUN-domain-containing protein; NOL1/NOP2/Sun domain family 2; NOL1/NOP2/Sun domain family member 2; NOL1/NOP2/Sun domain family, member 2; NOP2/Sun domain family, member 2; NOP2/Sun RNA methyltransferase 2; NOP2/Sun RNA methyltransferase family member 2; NSUN2; RNA cytosine C(5)-methyltransferase NSUN2; SAKI; substrate of AIM1/Aurora kinase B; TRM4; tRNA (cytosine-5-)-methyltransferase NSUN2; tRNA (cytosine(34)-C(5))-methyltransferase; tRNA cytosine C(5)-methyltransferase; tRNA methyltransferase 4 homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG