CST, 36106S, CDK10 (D8Z7Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CDK10. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CDK10 (D8Z7Z) Rabbit Monoclonal Antibody (CST #36106) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CDK10 (D8Z7Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDK10 protein. This antibody is not expected to react with CDK10 N-terminal deletion isoforms. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val24 of human CDK10 protein. Background Cyclin Dependent Kinase 10 (CDK10) is a Cdc2-related protein kinase that binds to and inhibits the transactivation activity of the transcription factor Ets2 (1). CDK10 is activated by cyclin M, which is mutated and unable to activate CDK10 in the human developmental disorder, STAR syndrome. Phosphorylation of Ets2 by CDK10/Cyclin M leads to degradation of Ets2 by the proteasome (2). CDK10 also plays a role in the development of the zebrafish nervous system (3). Studies have shown that expression of CDK10, which is modulated by promoter hypermethylation, is decreased in human cancer (4-6). Further, studies show that CDK10 expression in breast cancer affects response to tamoxifen (7), and is correlated with disease progression (8). CDK10 regulates the expression of c-RAF, and signaling through the MAPK pathway (2-3, 6-7). Alternate Names ALSAS; CDC2-related protein kinase; CDK10; Cell division protein kinase 10; cyclin dependent kinase 10; cyclin-dependent kinase (CDC2-like) 10; Cyclin-dependent kinase 10; cyclin-dependent kinase related protein; PISSLRE; serine/threonine protein kinase PISSLRE; Serine/threonine-protein kinase PISSLRE Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG