CST, 36135T, POU2F3 (E5N2D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying OCT11/POU2F3. Validated for WB,IHC,IHC,ChIP,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, POU2F3 (E5N2D) Rabbit Monoclonal Antibody (CST #36135) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 IHC Leica Bond: 1:100 - 1:400 Immunohistochemistry (Paraffin): 1:50 - 1:200 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 CUT&RUN: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 38561 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Chromatin IP, Chromatin IP-seq, CUT&RUN Specificity / Sensitivity POU2F3 (E5N2D) Rabbit Monoclonal Antibody recognizes endogenous levels of total POU2F3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu160 of human POU2F3 protein. Background POU2F3 (also known as OCT-11 and SKN-1) belongs to the OCT family of octamer motif-binding transcription factors, members of which play varied, diverse roles in animal development. POU2F3 is a lineage-defining transcription factor, responsible for driving differentiation of specialized chemosensory cells in the gastrointestinal and respiratory tracts (1-3). Tuft cells are a subtype of chemosensory cell, defined by POU2F3 expression, and which are hypothesized to be the origin of a distinct, non-neuroendocrine subtype of small cell lung cancer (SCLC) known as tuft cell-like SCLC (4). Consistent with its lineage-defining role in tuft cell development, POU2F3 was identified through a Crispr/Cas9 screen as a master regulator of tuft cell-like SCLC (5). Alternate Names Epoc-1; FLJ40063; MGC126698; Oct-11; OCT11; Octamer-binding protein 11; Octamer-binding transcription factor 11; OTF-11; OTF11; PLA-1; PLA1; PO2F3; POU class 2 homeobox 3; POU domain transcription factor OCT11a; POU domain, class 2, transcription factor 3; POU transcription factor; POU2F3; Skn-1a; Transcription factor PLA-1; Transcription factor Skn-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 45-60 Source/Isotype: Rabbit IgG