CST, 36193T, CD3 gamma (F1D9A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD3G. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CD3 gamma (F1D9A) Rabbit Monoclonal Antibody (CST #36193) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CD3 gamma (F1D9A) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD3γ protein. This antibody does not cross-react with CD3δ protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CD3γ protein. Background When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5). CD3γ is one of the invariant chains of the TCR signaling complex and is expressed at the cell surface as a type-I transmembrane glycoprotein, which forms a non-covalent heterodimer with CD3ε. Like other invariant chains of the TCR signaling complex, CD3γ contains a single ITAM with two embedded tyrosine residues that are likely directly phosphorylated by members of the Src family of tyrosine kinases to facilitate T cell activation (6,7). Research studies have demonstrated that CD3γ is also phosphorylated on serine residues, constituting a mechanism to downregulate TCR signaling through receptor internalization (8). Alternate Names CD3-GAMMA; CD3G; CD3G antigen, gamma polypeptide; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); FLJ17620; FLJ17664; FLJ79544; FLJ94613; IMD17; MGC138597; T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 gamma chain; T-cell surface glycoprotein CD3 gamma chain; T3G Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25, 20 Source/Isotype: Rabbit IgG