CST, 36214SF, Phospho-IKK alpha/beta (Ser176/180) (16A6) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying IKK-alpha (Ser176/Ser180) phosphate. Validated for WB,IHC,F,ELISA+. Highly specific and rigorously validated in-house, Phospho-IKK alpha/beta (Ser176/180) (16A6) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #36214) is ready to ship. Product Usage Information This product is the carrier free version of product #2697. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2697 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-IKK alpha/beta (Ser176/180) (16A6) Rabbit Monoclonal Antibody (BSA and Azide Free) detects IKKα only when phosphorylated at Ser176/180 and IKKβ only when phosphorylated at Ser177/181. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα. Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13). Alternate Names CHUK; component of inhibitor of nuclear factor kappa B kinase complex; conserved helix-loop ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; IkappaB kinase; IkB kinase alpha subunit; IkBKA; IKK-A; IKK-a kinase; IKK-alpha; IKK1; IKKA; Inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; TCF-16; TCF16; Transcription factor 16 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 85 IKK-alpha 87 IKK-beta Source/Isotype: Rabbit IgG