CST, 3622S, Presenilin 1 Antibody

Polyclonal Antibody for studying Presenilin-1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Presenilin 1 Antibody (CST #3622) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Presenilin 1 Antibody detects endogenous levels of the carboxy-terminal fragment of presenilin 1 (~22 kDa). This antibody detects the full length protein (55k Da) to a lesser extent. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near valine 293 of human presenilin 1. Antibodies are purified by protein A and peptide affinity chromatography. Background Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer disease, probably due to presenilin's associated γ-secretase activity for amyloid-β protein processing (1,2). Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) (2,3). Alternate Names ACNINV3; AD3; FAD; presenilin 1; Presenilin-1; Presenilin-1 CTF subunit; Presenilin-1 CTF12; Presenilin-1 NTF subunit; Protein S182; PS-1; PS1; PS1-CTF12; PSEN1; PSN1; PSNL1; S182 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 22 (C-terminal fragment). 55 (full-length). SOURCE: Rabbit