CST, 3625T, NUT (C52B1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NUT1. Validated for WB,IP,IHC,IF. Available in 3 sizes. Highly specific and rigorously validated in-house, NUT (C52B1) Rabbit Monoclonal Antibody (CST #3625) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:800 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 64162 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity NUT (C52B1) Rabbit Monoclonal Antibody detects endogenous levels of total NUT protein. This antibody also detects endogenous levels of the BRD4-NUT fusion protein found in NUT midline carcinoma (NMC). Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the human NUT protein. Background Nuclear protein in testis (NUT) is normally confined to the germ cells of the testis and ovary (1,2). NUT midline carcinoma (NMC) is a recently recognized cancer that is defined by the presence of chromosomal rearrangements involving the gene on chromosome 15q14 (3). In most cases the chromosomal translocation occurs between NUT and BRD4 on chromosome 19, resulting in the formation of a BRD4-NUT fusion protein. In the remaining tumors, variant NUT rearrangements are present involving BRD3, a very close homolog of BRD4. BRD4-NUT and BRD3-NUT encode fusion proteins that appear to contribute to carcinogenesis by blocking epithelial cell differentiation. NMCs, which are aggressive and highly lethal carcinomas, are morphologically indistinguishable from other poorly differentiated carcinomas. Given the limited expression of endogenous NUT protein, this antibody can be used to detect NUT fusion proteins in tissues by immunohistochemistry and immunofluorescence (2). Alternate Names C15orf55; DKFZp434O192; FAM22H; MGC138683; MGC138684; Nuclear protein in testis; NUT; NUT family member 1; NUT midline carcinoma family member 1; NUTM1; Protein NUT Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG