CST, 36276S, COL12A1 (F4D4U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying COL12A1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, COL12A1 (F4D4U) Rabbit Monoclonal Antibody (CST #36276) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity COL12A1 (F4D4U) Rabbit Monoclonal Antibody recognizes endogenous levels of total COL12A1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human COL12A1 protein. Background The extracellular matrix (ECM) is a complex network of macromolecules that provides structural tissue support to cells in the basement membrane and interstitial matrix. It is composed of many molecules, including proteins, glycoproteins, proteoglycans, and polysaccharides (1,2). One of the major proteins that comprise the ECM, and the human body, is collagen. Collagens are a large family of proteins. They are trimeric molecules composed of three alpha polypeptide chains that form a triple helix structure that is characteristic of all collagens (3). The large family of collagens is divided into three subgroups: fibrillar collagens, non-fibril forming collagens, and fibril-associated collagens. These subgroups differ in their structure and supramolecular assembly (3). Collagen type XII α1 chain (COL12A1), the largest member of the fibril-associated collagens with interrupted triple helix (FACIT) collagen family, contains fibronectin type III repeats, von Willebrand factor A domains, and two triple-helical domains (4). There are two major isoforms, a long form of 3063 amino acids and a short form of 1899 amino acids. COL12A1 binds to type I collagen fibrils and contributes to tissue biomechanics in the cornea, skeletal muscle, and tendon tissues (reviewed in 5). Mutations in the gene are correlated with myopathapies (6,7). COL12A1 expression is upregulated in several types of cancer; this elevation is thought to enhance tumor migration, invasiveness, and metastasis and, in some cases, correlates with poor prognosis (8-13). Alternate Names BA209D8.1; BTHLM2; COCA1; COL12A1; COL12A1L; Collagen alpha-1(XII) chain; collagen type XII alpha 1 chain; collagen type XII proteoglycan; collagen, type XII, alpha 1; collagen, type XII, alpha 1-like; DJ234P15.1; EDSMYP; UCMD2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 333 Source/Isotype: Rabbit IgG