CST, 36281S, IMPDH2 Antibody

Polyclonal Antibody for studying IMPDH2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, IMPDH2 Antibody (CST #36281) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity IMPDH2 Antibody recognizes endogenous levels of total IMPDH2 protein. This antibody does not cross-react with IMPDH1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala419 of human IMPDH2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Inosine-5'-monophosphate dehydrogenase (IMPDH) catalyzes the conversion of IMP to XMP, the first and rate-limiting step of guanine nucleotide biosynthesis (1). The isozymes IMPDH1 and IMPDH2 are encoded by two distinct genes (2). Research studies show that IMPDH2 is overexpressed in glioblastoma. In glioblastoma, IMPDH2-mediated GTP biosynthesis is essential for rRNA and tRNA synthesis and the formation of enlarged and irregular nucleoli. In addition, and double knockout leads to significant inhibition of tumor growth in an orthotopic glioblastoma model (3). Furthermore, IMPDH1 and IMPDH2 expression is upregulated in small cell lung cancer cells with low expression of ASCL1 transcription factor (ASCL1 ). Suppression of IMPDH activity inhibits ASCL1 small cell lung cancer growth (4). Alternate Names epididymis secretory sperm binding protein; IMDH2; IMP (inosine 5'-monophosphate) dehydrogenase 2; IMP (inosine monophosphate) dehydrogenase 2; IMP dehydrogenase 2; IMP oxireductase 2; IMPD 2; IMPD2; IMPDH 2; IMPDH-II; IMPDH2; inosine 5' phosphate dehydrogenase 2; inosine monophosphate dehydrogenase 2; inosine monophosphate dehydrogenase type II; Inosine-5'-monophosphate dehydrogenase 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit