CST, 3632S, Nicastrin Antibody

Polyclonal Antibody for studying Nicastrin. Validated for Western Blotting. Highly specific and rigorously validated in-house, Nicastrin Antibody (CST #3632) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Nicastrin Antibody detects endogenous levels of Nicastrin. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human Nicastrin. Antibodies are purified by protein A and peptide affinity chromatography. Background Nicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (1,2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (3). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to γ-secretase to ensure the correct cleavage process (2,4). Alternate Names anterior pharynx-defective 2; APH2; ATAG1874; KIAA0253; NCSTN; NICA; Nicastrin Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110, 120 SOURCE: Rabbit