CST, 36406S, Synaptophysin (D8F6H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SYP. Validated for WB,WB,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Synaptophysin (D8F6H) Rabbit Monoclonal Antibody (CST #36406) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 25056 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Synaptophysin (D8F6H) Rabbit Monoclonal Antibody recognizes endogenous levels of total Synaptophysin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly299 of human Synaptophysin protein. Background Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4). Alternate Names Major synaptic vesicle protein p38; MRX96; MRXSYP; Synaptophysin; SYP; SYPH Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Rabbit IgG