CST, 36425S, Cleaved Gasdermin D (Asp275) (E7H9G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying gasdermin D (Asp275) cleaved. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 3 sizes. Highly specific and rigorously validated in-house, Cleaved Gasdermin D (Asp275) (E7H9G) Rabbit Monoclonal Antibody (CST #36425) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:500 - 1:2000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 37349 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Cleaved Gasdermin D (Asp275) (E7H9G) Rabbit Monoclonal Antibody recognizes endogenous levels of the amino fragment of human gasdermin D protein only when cleaved at Asp275. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp275 of human Gasdermin D protein. Background Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7). Alternate Names DF5L; DFNA5L; FKSG10; FLJ12150; gasdermin D; gasdermin domain containing 1; Gasdermin domain-containing protein 1; Gasdermin-D; Gasdermin-D, C-terminal; Gasdermin-D, N-terminal; GSDMD; GSDMD-CT; GSDMD-NT; GSDMDC1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG