CST, 36467S, CCL5/RANTES (E9S2K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CCL5. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CCL5/RANTES (E9S2K) Rabbit Monoclonal Antibody (CST #36467) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:150 - 1:600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 57219 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CCL5/RANTES (E9S2K) Rabbit Monoclonal Antibody recognizes endogenous levels of total CCL5/RANTES protein. This antibody recognizes human CCL5/RANTES protein and is also reactive with mouse CCL5/RANTES; however, this antibody is not suggested for immunohistochemical analysis of mouse tissues. Non-specific staining was observed in human pancreatic islets by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with human CCL5/RANTES recombinant protein. Background RANTES/CCL5 (regulated upon activation, T cell expressed and secreted) is a member of the C-C or β family of chemokines that induce inflammation and are associated with a number of inflammatory disorders (1,2). RANTES is produced and secreted mainly by CD8 T cells, macrophages, and platelets, as well as epithelial cells, fibroblasts, and some solid tumors (2-7). RANTES acts as a chemoattractant and has other regulatory functions on a number of cell types, including monocytes, memory T cells, NK cells, eosinophils, basophils, dendritic cells, and mast cells (3, 7-9). Signaling by RANTES is mediated by several G protein-coupled receptors (GPCRs), including CCR1, CCR3, CCR4, and CCR5. Alternate Names beta-chemokine RANTES; C-C motif chemokine 5; C-C motif chemokine ligand 5; CCL5; chemokine (C-C motif) ligand 5; D17S136E; EoCP; Eosinophil chemotactic cytokine; MGC17164; RANTES; RANTES(3-68); RANTES(4-68); regulated upon activation, normally T-expressed, and presumably secreted; SCYA5; SIS-delta; SISd; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG