CST, 36471SF, BRD9 (E9R2I) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying BRD9. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, BRD9 (E9R2I) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #36471) is ready to ship. Product Usage Information This product is the carrier free version of product #58906. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 58906 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity BRD9 (E9R2I) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total BRD9 protein. This antibody does not cross-react with BRD7 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human BRD9 protein. The epitope has been mapped to residues surrounding Asp347. Background ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes, such as gene expression, DNA replication, and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors, such as nuclear receptors, p53, RB, and BRCA1, to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1,6-9). GLTSCR1 and its paralog GLTSCR1L, along with BRD9, have been identified as unique subunits of the non-canonical BAF (ncBAF) or GBAF complex. This complex contains either GLTSCR1 or GLTSCR1L instead of an ARID subunit, while also lacking the BAF45, BAF47, and BAF57 subunits. GBAF maps to regions distinct from the PBAF and canonical BAF complexes and has also been shown to be a synthetic lethal target for BAF-driven cancers, such as synovial sarcomas and rhabdoid tumors (10-12). Alternate Names BRD9; bromodomain containing 9; Bromodomain-containing protein 9; DKFZp434D0711; DKFZp686L0539; FLJ43530; LAVS3040; PRO9856; Rhabdomyosarcoma antigen MU-RMS-40.8; sarcoma antigen NY-SAR-29 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG