CST, 36556S, TIA-1 (F6V4I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TIA1 mouse. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, TIA-1 (F6V4I) Rabbit Monoclonal Antibody (CST #36556) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TIA-1 (F6V4I) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIA-1 protein. This antibody shows some cross-reactivity with human TIAR protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse TIA-1 protein. Background T-cell intracellular antibody 1 (TIA-1) is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins that was originally found to induce DNA fragmentation in digitonin-permeabilized thymocytes (1). TIA-1 protein has about 80% identity to the related TIAR protein, both of which possess three amino-terminal RRM domains and a glutamine-rich carboxyl terminus (1,2). Alternative splicing is responsible for generating at least two isoforms of TIA-1 and TIAR (3,4). Several research studies indicate that TIA-1 and TIAR play a role in apoptosis, cellular stress, and inflammation. Importantly, TIA-1 and TIAR translocate from the nucleus to stress granules in response to a variety of environmental stresses (5-8). Stress granules function as sites of translational repression in response to potentially damaging conditions. mRNA transcripts targeted by TIA-1 and TIAR include TNF-α, Cox2, cytochrome c, GADD45α, and HIF-1α (8-13). Alternate Names 2310050N03Rik; AI256674; Cytotoxic granule associated RNA binding protein TIA1; cytotoxic granule-associated RNA binding protein 1; cytotoxic granule-associated RNA-binding protein 1; mTIA; mTIA-1; Nucleolysin TIA-1; OTTMUSP00000026222; RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; Tia; TIA-1; Tia1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 42, 44 Source/Isotype: Rabbit IgG